Render Target: STATIC
Render Timestamp: 2024-12-06T10:39:39.137Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-30 01:54:27.196
Product last modified at: 2024-10-17T14:15:14.806Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Orexin (D6G9T) Rabbit mAb #16743

Filter:
  • IHC
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous (IHC-P, IF-F), Transfected (W)
    MW (kDa) 16
    Source/Isotype Rabbit IgG
    Application Key:
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:100
    Immunofluorescence (Frozen) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Orexin (D6G9T) Rabbit mAb recognizes endogenous levels of total orexin protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the sequence of the mature human orexin protein.

    Background

    Orexin, also called HCRT or hypocretin, is expressed as a precursor that is processed into two biologically active neuropeptides called orexin-A (HCRT1) and orexin-B (HCRT2) (1). Orexin is expressed in the lateral and posterior hypothalamus and plays a role in feeding behavior (1). The orexin neuropeptides act by stimulating the two orexin receptors that belong to the G Protein-Coupled Receptors family (1). Orexin receptor 1 is mostly found in the hypothalamic region, while orexin receptor 2 is also found in the cerebral cortex and the nucleus accumbent (2). Orexin also plays a role in adipocyte homeostasis and is required for brown adipose tissue development, differentiation, and function (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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