Render Target: STATIC
Render Timestamp: 2024-10-11T10:00:14.475Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 02:00:07.678
Product last modified at: 2024-09-30T08:00:22.443Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

OTUD6B (F6K7V) Rabbit mAb #90190

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 34
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    OTUD6B (F6K7V) Rabbit mAb recognizes endogenous levels of total OTUD6B protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala101 of human OTUD6B protein.

    Background

    Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM (1,2). The OTU subfamily comprises a group of approximately 100 putative cysteine proteases that are homologous to the ovarian tumor gene product of Drosophila (3). OTU domain-containing protein 6B (OTUD6B) is a member of the OTU family that has active deubiquitinase activity and plays a role in various biological processes (4,5). OTUD6B has been shown to regulate protein synthesis operating downstream of mTORC1 through its two major splicing isoforms, OTUD6B-1 and OTUD6B-2, which target different substrates (6). The switch from OTUD6B-1 to OTUD6B-2 expression may promote protein synthesis and cell proliferation in non-small cell lung cancer (NSCLC) cells (6). OTUD6B is generally upregulated in other types of cancer and is associated with poor prognosis, suggesting that it behaves as an oncogene (7). Alternatively, OTUD6B has been reported to act as a tumor suppressor in hepatocellular carcinoma (HCC) via deubiquitination of VHL and repression of HIF-1α (8). OTUD6B positively regulates type I interferon (IFN) immune response by deubiquitinating and stabilizing the transcription factor IRF3 at Lys315, and overexpression of OTUD6B in mice enhances IFN response following viral infection (9). Additionally, biallelic loss-of-function mutations in the OTUD6B gene have been shown to cause an intellectual disability syndrome that is characterized by cognitive dysfunction and seizures (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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