OX40L (D6K7R) Rabbit mAb (BSA and Azide Free) #51635
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #59036. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
OX40L (D6K7R) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total OX40L protein. Staining of unknown specificity has been observed in skeletal muscle.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala15 of human OX40L protein.
Background
OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12).
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- Dürkop, H. et al. (1995) Br J Haematol 91, 927-31.
- Godfrey, W.R. et al. (1994) J Exp Med 180, 757-62.
- Al-Shamkhani, A. et al. (1997) J Biol Chem 272, 5275-82.
- So, T. et al. (2011) Proc Natl Acad Sci U S A 108, 2903-8.
- So, T. and Croft, M. (2013) Front Immunol 4, 139.
- Gough, M.J. and Weinberg, A.D. (2009) Adv Exp Med Biol 647, 94-107.
- Weinberg, A.D. et al. (2011) Immunol Rev 244, 218-31.
- Linch, S.N. et al. (2015) Front Oncol 5, 34.
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