Render Target: STATIC
Render Timestamp: 2024-11-08T10:02:18.298Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:59:48.996
Product last modified at: 2024-09-30T08:02:06.892Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PADI4 (F1G7E) Rabbit mAb #56308

Filter:
  • WB
  • ChIP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 68
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Chromatin IP 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PADI4 (F1G7E) Rabbit mAb recognizes endogenous levels of total PADI4 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human PADI4 protein.

    Background

    Peptidyl arginine deiminase (PAD) proteins are a family of Ca2+-dependent enzymes that catalyze the post-translational conversion of arginine to citrulline. There are currently five known PAD isozymes in humans, referred to as PADI1-4 and PADI6 (1). PADI4 is a nuclear member of the PAD family and is responsible for deiminating R2, R8, R17, and R26 on the histone H3 (2-4). Deimination of histone residues prevents CARM1-mediated arginine methylation and results in transcriptional repression (2,3). Expression of PADI4 is increased during myeloid differentiation and various polymorphisms have been implicated in rheumatoid arthritis (5-8). PADI4 can play a role in apoptosis and cell cycle arrest upon DNA damage, as it is recruited to the p21 promoter by p53 and also regulates a subset of p53 target genes (9,10). Hypercitrullination of histones by PADI4 contributes to chromatin decondensation and the formation of neutrophil extracellular traps (NETs) to combat bacterial infection in a process called NETosis (11,12). Inhibition of PADI4 reduces NET formation, making it a potential therapeutic target (13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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