Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PAR1 (E9J9L) Rabbit Monoclonal Antibody #79109

Filter:
  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 47, 70-110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PAR1 (E9J9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAR1 protein. This antibody recognizes a background band at 24 kDa of unknown origin.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp50 of human PAR1 protein.

    Background

    PAR1 (F2R) belongs to the PAR (Protease-Activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through amino terminal cleavage of the receptor by serine proteases such as thrombin, trypsin, or matrix metalloproteinases (1,2). Protease-mediated cleavage exposes the ‘tethered-ligand’ fragment of the receptor; this binds to a second extracellular loop of the receptor and triggers receptor activation. Activated PAR1 couples to distinct G-protein complexes or arrestins, further relaying signals to downstream players (e.g., phospholipase C, Rho-kinase) (3-4). PAR1 function is necessary for normal physiological processes such as hemostasis, platelet activation, and thrombosis, whereas abnormal activation of PAR1 has been associated with inflammatory disorders, cardiovascular diseases, and metastasis (4-6).

    Alternate Names

    CF2R; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; coagulation factor II thrombin receptor; F2R; HTR; PAR-1; PAR1; protease-activated receptor 1; Proteinase-activated receptor 1; Thrombin receptor; TR

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.