Pathological Hallmarks of Alzheimer's Disease Antibody Sampler Kit #74113
Product Information
Kit Usage Information
Protocols
- 7074: Western Blotting
- 8243: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence
- 12885: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence
- 12990: Western Blotting, Immunofluorescence
- 14974: Western Blotting, Immunofluorescence
- 14975: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence
- 20194: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Immunofluorescence
- 32098: Western Blotting, Immunofluorescence
- 46687: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence
- 49561: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence
Product Description
Background
Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (4). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (4). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (4). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (5-8). Aβ-43 has been suggested as a biomarker in early onset of Alzheimer's disease, where patients have lower levels of Aβ-43 in cerebrospinal fluid (8-10). Several studies have shown that Aβ toxicity of Aβ-43 is as high as Aβ-42 or Aβ-40 in different models of Alzheimer's disease, including mouse models and human disease (10).
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- Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
- Mitchell, A.J. (2009) J Neurol Neurosurg Psychiatry 80, 966-75.
- Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
- Caporaso, G.L. et al. (1992) Proc Natl Acad Sci U S A 89, 3055-9.
- Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
- Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
- Ando, K. et al. (1999) J Neurosci 19, 4421-7.
- Iijima, K. et al. (2000) J Neurochem 75, 1085-91.
Limited Uses
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