Render Target: STATIC
Render Timestamp: 2025-02-13T11:03:51.897Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2024-09-17 16:01:11.787
Product last modified at: 2025-01-01T09:04:52.445Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

PAX8 (BC12) Mouse mAb #28556

Filter:
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Storage

    Supplied in a Tris-based buffer with ~1% BSA and less than 0.1% sodium azide. Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PAX8 (BC12) Mouse mAb recognizes endogenous levels of PAX8 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with the full-length human recombinant PAX8 protein.

    Background

    Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).
    PAX8 is involved in the development of thyroid follicular cells and the expression of thyroid-specific genes (5). Investigators have associated mutations in the PAX8 gene with thyroid dysgenesis, thyroid follicular carcinomas, and atypical follicular thyroid adenomas (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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