Render Target: STATIC
Render Timestamp: 2025-03-21T10:22:33.913Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:20:03.849
Product last modified at: 2025-01-17T19:00:10.938Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PCM-1 (F8Q8B) Rabbit mAb #53705

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 228
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Frozen) 1:400 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PCM-1 (F8Q8B) Rabbit mAb recognizes endogenous levels of total PCM-1 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PCM-1 protein.

    Background

    The centrosome is composed of a pair of centrioles surrounded by electron-dense pericentriolar material and functions as the microtubule-organizing center responsible for microtubule nucleation and spindle organization during cell cycle progression (1). Pericentriolar material 1 (PCM-1) is a large, 228 kDa protein associated with the centrosome in a cell cycle-dependent manner (2). PCM-1 localizes to small cytoplasmic granules called centriolar satellites (3). PCM-1 is required for the assembly of several centrosomal proteins, including centrin, pericentrin, ninein, NEK2, and CEP250 (4-8). Chromosomal translocations involving genes encoding PCM-1 and the tyrosine kinases Ret and Jak2 are associated with some cancers, including papillary thyroid carcinoma and myeloid leukemia (9-11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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