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PhosphoSitePlus® Resource

  • Additional protein information
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We recommend the following alternatives

W IP IHC   F   H XP
REACTIVITY SENSITIVITY MW (kDa) Isotype
40-50 Rabbit 

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

PD-1 (D3W4U) Rabbit mAb recognizes endogenous levels of total PD-1 protein. This antibody binds the intracellular domain of human PD-1 protein.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala249 of human PD-1 protein.

The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).


1.  Ishida, Y. et al. (1992) EMBO J 11, 3887-95.

2.  Shinohara, T. et al. (1994) Genomics 23, 704-6.

3.  Nishimura, H. et al. (1999) Immunity 11, 141-51.

4.  Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.

5.  Yokosuka, T. et al. (2012) J Exp Med 209, 1201-17.

6.  Sheppard, K.A. et al. (2004) FEBS Lett 574, 37-41.

7.  Chemnitz, J.M. et al. (2004) J Immunol 173, 945-54.

8.  Thibult, M.L. et al. (2013) Int Immunol 25, 129-37.

9.  Dong, H. et al. (2002) Nat Med 8, 793-800.

10.  Topalian, S.L. et al. (2012) Curr Opin Immunol 24, 207-12.


Entrez-Gene Id 5133
Swiss-Prot Acc. Q15116


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.

15121
PD-1 (D3W4U) Rabbit mAb