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85095
PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) (BSA and Azide Free) #85095

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  1. IHC
Immunohistochemistry Image 1: PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal mouse thymus using PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal mouse lung using PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded RAW 264.7 cell pellet (left, negative) or mouse bone marrow-derived macrophages cell pellet (right, positive) using PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific). Data were generated using the standard formulation of this product.
To Purchase # 85095
Cat. # Size Qty. Price
85095SF
100 µg $ 746

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #64988. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #64988

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) (BSA and Azide Free) recognizes endogenous levels of total mouse PD-L1 protein. Non-specific staining of keratinized epithelium has been observed.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly216 of mouse PD-L1 protein.

Background

Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor-infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9).
  1. Dong, H. et al. (1999) Nat Med 5, 1365-9.
  2. Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
  3. Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16.
  4. Dong, H. et al. (2002) Nat Med 8, 793-800.
  5. Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5.
  6. Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64.
  7. Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37.
  8. Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41.
  9. Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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