PD-L2 (D6L5A) Rabbit mAb #49189
- IHC
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #64714.
Protocol
Specificity / Sensitivity
PD-L2 (D6L5A) Rabbit mAb recognizes endogenous levels of total PD-L2 protein. Staining of unknown specificity in mouse ovary has been observed.
Species Reactivity:
Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala85 of mouse PD-L2 protein.
Background
Programmed cell death 1 ligand 2 (PD-L2, B7-DC, CD273) is a member of the B7 family of cell surface ligands that regulate T-cell activation and immune responses (1,2). PD-L2 binds the PD-1 transmembrane receptor and inhibits T-cell activation. PD-L2 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by activated dendritic cells, macrophages, and T-cells (1,3). Similar in structure to related B7 family members, PD-L2 protein contains extracellular IgV and IgC2 domains, a transmembrane domain, and a short, cytoplasmic region. Research studies demonstrate that PD-L2 is expressed in several tumor types, including lung cancer, renal cell carcinoma, melanoma, Hodgkin's lymphoma and primary mediastinal large B-cell lymphoma (4-7).
- Latchman, Y. et al. (2001) Nat Immunol 2, 261-8.
- Tseng, S.Y. et al. (2001) J Exp Med 193, 839-46.
- Messal, N. et al. (2011) Mol Immunol 48, 2214-9.
- Kim, M.Y. et al. (2015) Lung Cancer 88, 24-33.
- Taube, J.M. et al. (2014) Clin Cancer Res 20, 5064-74.
- Green, M.R. et al. (2010) Blood 116, 3268-77.
- Ansell, S.M. et al. (2015) N Engl J Med 372, 311-9.
Limited Uses
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