PD-L2 (D7U8C™) XP® Rabbit mAb (BSA and Azide Free) #99337
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #82723. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
PD-L2 (D7U8C™) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total PD-L2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg168 of human PD-L2 protein.
Background
Programmed cell death 1 ligand 2 (PD-L2, B7-DC, CD273) is a member of the B7 family of cell surface ligands that regulate T-cell activation and immune responses (1,2). PD-L2 binds the PD-1 transmembrane receptor and inhibits T-cell activation. PD-L2 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by activated dendritic cells, macrophages, and T-cells (1,3). Similar in structure to related B7 family members, PD-L2 protein contains extracellular IgV and IgC2 domains, a transmembrane domain, and a short, cytoplasmic region. Research studies demonstrate that PD-L2 is expressed in several tumor types, including lung cancer, renal cell carcinoma, melanoma, Hodgkin's lymphoma and primary mediastinal large B-cell lymphoma (4-7).
- Latchman, Y. et al. (2001) Nat Immunol 2, 261-8.
- Tseng, S.Y. et al. (2001) J Exp Med 193, 839-46.
- Messal, N. et al. (2011) Mol Immunol 48, 2214-9.
- Kim, M.Y. et al. (2015) Lung Cancer 88, 24-33.
- Taube, J.M. et al. (2014) Clin Cancer Res 20, 5064-74.
- Green, M.R. et al. (2010) Blood 116, 3268-77.
- Ansell, S.M. et al. (2015) N Engl J Med 372, 311-9.
Limited Uses
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