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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Pdx1 (D59H3) XP® Rabbit mAb #5679

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:50
    Immunofluorescence (Frozen) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #68797.

    Protocol

    Specificity / Sensitivity

    Pdx1 (D59H3) XP® Rabbit mAb detects endogenous levels of total Pdx1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val274 of human Pdx1 protein.

    Background

    The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (1,2). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (2,3). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (3), where it promotes the expression of genes important for β-cell functions such as insulin, glucokinase, and Glut2 (4-6). Mutations of the corresponding Pdx1 gene may be associated with diabetes and cases of pancreatic insufficiency (7).

    Alternate Names

    GSF; IDX; IDX-1; Insulin; Insulin promoter factor 1; Insulin upstream factor 1; IPF; IPF-1; IPF1; Islet/duodenum homeobox-1; IUF; IUF-1; MODY4; Pancreas/duodenum; Pancreas/duodenum homeobox protein 1; pancreatic and duodenal homeobox 1; PDX; PDX-1; PDX1; somatostatin transcription factor 1; STF; STF-1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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