Render Target: STATIC
Render Timestamp: 2025-03-19T11:32:32.331Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:11:20.910
Product last modified at: 2025-02-14T19:45:08.554Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PEAK1 (D4G6J) Rabbit mAb #72908

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 250
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PEAK1 (D4G6J) Rabbit mAb recognizes endogenous levels of total PEAK1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr695 of human PEAK1 protein.

    Background

    PEAK1 (Pseudopodium-enriched atypical kinase 1 or sgk269) is a member of nonreceptor atypical tyrosine kinase family identified by MS analysis of purified psedopodium (1). PEAK1 is a multi-domain protein with a N-terminal Erk binding site, followed by actin-targeting/Src substrate/Erk substrate region, Crk binding site, Shc binding site, and a C-terminal kinase domain (1, 2). By interacting with different adaptors like Shc, Grb2, Src, and others, PEAK1 functions as an important regulator in different signaling pathways, namely the Src/PEAK1/ErbB2 (3), EGFR Shc1/PEAK1/Grb2(4), TGFβ/PEAK1/Src/MAPK (5), and fibronectin/PEAK1/Src (6) pathways. PEAK1 plays an instrumental role in a wide variety of biological processes including epithelial-mesenchymal transition (EMT), dynamics of focal adhesion, cancer metastatic growth and invasion as well as cancer drug resistance (3, 5-8). Phosphorylation of PEAK1 at Tyr665 or Tyr635 by SFK (Src family Kinases) has been shown to be essential for cancer cell migration and invasion as well as the turnover of focal adhesions (7, 9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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