Western blot analysis of extracts from AR42J cells untreated (-) or treated with 1 μM thapsigargin (Tg) for 20 minutes (+), using Phospho-PERK (Thr980) (16F8) Rabbit mAb.Learn more about how we get our images
Western blot analysis of extracts from various cell types using PERK (C33E10) Rabbit mAb.Learn more about how we get our images
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.
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|12185S||1 Kit (10 western blots)||$489.00.0|