Interested in promotions? | Click here >>
39527
PhosphoPlus® PFKFB2 (Ser483) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® PFKFB2 (Ser483) Antibody Duet #39527

Reviews ()
Citations (0)
PhosphoPlus® PFKFB2 (Ser483) Antibody Duet: Image 1
Western blot analysis of extracts from LNCaP, Hep G2, and MCF7 cells using PFKFB2 (D5I5F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
PhosphoPlus® PFKFB2 (Ser483) Antibody Duet: Image 2
Immunoprecipitation of PFKFB2 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PFKFB2 (D5I5F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PFKFB2 (D5I5F) Rabbit mAb. An anti-rabbit IgG light chain antibody was used as the secondary antibody.
PhosphoPlus® PFKFB2 (Ser483) Antibody Duet: Image 3
Confocal immunofluorescent analysis of LNCaP (high expression, left) and HepG2 (low expression, right) using PFKFB2 (D5I5F) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
PhosphoPlus® PFKFB2 (Ser483) Antibody Duet: Image 4
Western blot analysis of extracts from serum starved HeLa cells, untreated (-) or treated with insulin (150 nM, 5 min; +) or IGF-1 (100 ng/ml, 5 min; +), using Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb (upper) or PFKFB2 (D7G5R) Rabbit mAb #13045 (lower). The phospho-specificity of Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb is verified by treating the nitrocellulose membranes with calf intestinal alkaline phosphatase (CIP) and λ protein phosphatase (λ PP) following transfer of proteins from the SDS-PAGE gel to the membranes.
To Purchase # 39527S
Product # Size Price
39527S
1 Kit $ 529

Product Includes Quantity Reactivity MW(kDa) Isotype
PFKFB2 (D5I5F) Rabbit mAb 13029 100 µl H Mk 55 Rabbit IgG
Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb 13064 100 µl H 55 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). Research studies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2 (3). In addition, androgen increases the expression of PFKFB2 in prostate cancer cells (4).
  1. Atsumi, T. et al. (2005) Diabetes 54, 3349-57.
  2. Kim, S.G. et al. (2006) J Biol Chem 281, 2939-44.
  3. Novellasdemunt, L. et al. (2013) J Biol Chem 288, 10640-51.
  4. Moon, J.S. et al. (2011) Biochem J 433, 225-33.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.