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Primary Antibodies /
Phospho-4E-BP1 (Thr46) Antibody

Phospho-4E-BP1 (Thr46) Antibody #9458

Inquiry Info. # 9458

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	15 to 20
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-4E-BP1 (Thr46) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr46. This antibody may cross-react with 4E-BP1 phosphorylated at Thr37, and with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr46 of mouse 4E-BP1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Alternate Names

4E-BP1; 4EBP1; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC4316; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1

Database Links

UniProt ID: Q13541

Entrez-Gene Id: 1978

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For Research Use Only. Not for Use in Diagnostic Procedures.

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