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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb 4060 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk Dm Z B 60 Rabbit IgG
Akt (pan) (C67E7) Rabbit mAb 4691 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk Dm 60 Rabbit IgG
Phospho-c-Raf (Ser259) Antibody 9421 40 µl
Western Blotting Immunoprecipitation
H M R Mk X 74 Rabbit 
Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb 5558 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M R Hm 46 Rabbit IgG
Phospho-PTEN (Ser380) Antibody 9551 40 µl
Western Blotting Immunoprecipitation
H M R 54 Rabbit 
Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb 3438 40 µl
Western Blotting Immunoprecipitation
H M R 58 to 68 Rabbit IgG
Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb 13038 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M R Mk 60 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Phospho-Akt Pathway Antibody Sampler Kit provides an economical means to evaluate the activation status of the Akt signaling pathway, including PTEN and phosphorylated Akt, GSK-3beta, c-Raf and PDK1. The kit includes enough primary and secondary antibodies to perform four Western blot experiments.


Specificity / Sensitivity

Each phospho-specific antibody recognizes the phosphorylated form of its target. Akt Antibody recognizes total Akt protein, independent of its phosphorylation state.


Source / Purification

Antibodies are produced by immunizing rabbits with synthetic phosphopeptides corresponding to residues surrounding Ser473 or Thr308 of human Akt, Ser9 of human GSK-3ß, Ser259 of human c-Raf, Ser380 of human PTEN or Ser241 of human PDK1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).


1.  Franke, T.F. et al. (1997) Cell 88, 435-7.

2.  Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.

3.  Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.

4.  Franke, T.F. et al. (1995) Cell 81, 727-36.

5.  Cross, D.A. et al. (1995) Nature 378, 785-9.

6.  Manning, B.D. et al. (2002) Mol Cell 10, 151-62.

7.  Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.

8.  Sarbassov, D.D. et al. (2005) Science 307, 1098-101.

9.  Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

10.  Jacinto, E. et al. (2006) Cell 127, 125-37.

11.  Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.

12.  Cardone, M.H. et al. (1998) Science 282, 1318-21.

13.  Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.

14.  Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.

15.  Brunet, A. et al. (1999) Cell 96, 857-68.

16.  Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.

17.  Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.

18.  Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.

19.  Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.


Entrez-Gene Id 207 , 208 , 10000 , 5894 , 2932 , 5170 , 5728
Swiss-Prot Acc. P31749 , P31751 , Q9Y243 , P04049 , P49841 , O15530 , P60484


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

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Phospho-Akt Pathway Antibody Sampler Kit