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Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) Antibody #3068

Inquiry Info. # 3068

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 35, 40, 48
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) Antibody detects endogenous levels of Aurora A/B/C only when phosphorylated at either Thr288, Thr232 or Thr198 respectively.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr232 of human Aurora B. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).

    Alternate Names

    AIE2; AIK; AIK2; AIK3; AIM-1; AIM1; AIRK1; AIRK2; AIRK3; ARK-1; ARK-2; ARK-3; ARK1; ARK2; ARK3; AURA; AurB; AurC; AURKA; AURKB; aurkb-sv1; aurkb-sv2; AURKC; Aurora 1; Aurora 2; Aurora 3; Aurora A; Aurora B; Aurora C; Aurora kinase A; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora kinase C; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-B; aurora-C; Aurora-related kinase 1; Aurora-related kinase 2; Aurora-related kinase 3; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; Aurora/IPL1-related kinase 2; Aurora/IPL1-related kinase 3; Aurora/IPL1/Eg2 protein 2; AURORA2; AYK1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; epididymis secretory protein Li 90; hARK1; HEL-S-90; IAK1; IPL1; IPL1-related kinase; MGC34538; PPP1R47; PPP1R48; protein phosphatase 1, regulatory subunit 47; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine kinase 13 (aurora/IPL1-like); serine/threonine kinase 6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase aurora-B; Serine/threonine-protein kinase aurora-C; SPGF5; STK-1; STK1; STK12; STK13; STK15; STK5; STK6; STK7

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