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Phospho-Bcl-2 (Ser70) Antibody (Human Specific) #2871

Inquiry Info. # 2871

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 28
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-Bcl-2 (Ser70) Antibody (Human Specific) detects endogenous levels of Bcl-2 only when phosphorylated at serine 70. This antibody does not cross-react with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser70 of human Bcl-2. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

    Alternate Names

    Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; BCL2; BCL2 apoptosis regulator; PPP1R50; protein phosphatase 1, regulatory subunit 50

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