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Phospho-CREB (Ser133) (1B6) Mouse mAb #5181

Inquiry Info. # 5181

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 43
    Source/Isotype Mouse

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-CREB (Ser133) (1B6) Mouse mAb can be used in high throughput kinase assays and drug discovery applications. This antibody detects CREB only when phosphorylated at Ser133. This antibody also detects the phosphorylated form of CREB-related protein ATF-1.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser133 of human CREB.

    Background

    CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
    Description: Cell Signaling Technology antibody formulated in PBS (no BSA/no glycerol) and quality controlled for use in ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists. Please inquire for bulk pricing of this antibody.

    Alternate Names

    active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein

    For Research Use Only. Not for Use in Diagnostic Procedures.
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