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64191
Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (BSA and Azide Free) #64191

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  1. WB
  2. IHC
Western Blotting Image 1: Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (BSA and Azide Free)
Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb specifically binds to phosphorylated M-CSF receptors, but not other phosphorylated protein tyrosine kinases. Western blot analysis of extracts from various cell lines expressing different activated protein tyrosine kinases, using Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (upper). The same membrane was stripped and reprobed with Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100) (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from NKM-1 cells, untreated or treated with an M-CSFR inhibitor, using Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (upper) or CSF-1R/M-CSF-R Antibody #3152 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded FDCP1/fms cells, untreated (left) or mCSF-treated (right), using Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 64191
Cat. # Size Qty. Price
64191SF
100 µg
$ 776

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 175
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #3155. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #3155.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (BSA and Azide Free) detects endogenous levels of CSF-1R/M-CSF-R only when phosphorylated at tyrosine 723. The antibody does not cross-react with related active protein tyrosine kinases.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr723 of human CSF-1R/M-CSF-R.

Background

Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8).
  1. Stanley, E.R. et al. (1978) Nature 274, 168-70.
  2. Byrne, P.V. et al. (1981) J Cell Biol 91, 848-53.
  3. Bourette, R.P. and Rohrschneider, L.R. (2000) Growth Factors 17, 155-66.
  4. Novak, U. et al. (1996) Oncogene 13, 2607-13.
  5. Bourette, R.P. et al. (1997) EMBO J 16, 5880-93.
  6. Morley, G.M. et al. (1999) Oncogene 18, 3076-84.
  7. Toy, E.P. et al. (2001) Gynecol Oncol 80, 194-200.
  8. Maher, M.G. et al. (1998) Clin Cancer Res 4, 1851-6.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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