Phospho-DAPP1/BAM32 (Tyr139) (D7G4G) Rabbit mAb #13703
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 29 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Research studies show that phosphorylation of DAPP1/BAM32 at Tyr139 is PI3K-dependent, requires an intact PH domain in DAPP1/BAM32, and is likely performed by Src-family kinases following membrane recruitment of DAPP1/BAM32 by phosphoinositides (6). Blocking phosphorylation of DAPP1/BAM32 at Tyr139 inhibits BCR internalization and reduces cellular F-actin levels, suggesting that phosphorylation of DAPP1/BAM32 may play a role in regulating actin-dependent internalization of the activated BCR (7,8).
- Marshall, A.J. et al. (2007) Biochem Soc Trans 35, 181-2.
- Marshall, A.J. et al. (2000) J Exp Med 191, 1319-32.
- Anderson, K.E. et al. (2000) Curr Biol 10, 1403-12.
- Richards, S. et al. (2008) Immunol Rev 224, 183-200.
- Ortner, D. et al. (2011) J Immunol 187, 3972-8.
- Dowler, S. et al. (2000) Biochem J 349, 605-10.
- Niiro, H. et al. (2004) J Immunol 173, 5601-9.
- Allam, A. et al. (2004) J Biol Chem 279, 39775-82.
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