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Primary Antibodies /
Phospho-DARPP-32 (Thr34) Antibody

Phospho-DARPP-32 (Thr34) Antibody #2304

Inquiry Info. # 2304

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Product Specifications

REACTIVITY
SENSITIVITY	Endogenous
MW (kDa)	32
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-DARPP-32 (Thr34) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34. This antibody does not cross-react with DARPP-32 phosphorylated at Thr75. This antibody may also detect PP-1 inhibitor.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr34 of human DARPP-32. Antibodies are purified by protein A and peptide affinity chromatography.

Background

DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).

Alternate Names

DARPP-32; DARPP32; dopamine and cAMP regulated phosphoprotein; dopamine and cAMP-regulated neuronal phosphoprotein 32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; PPP1R1B; PPR1B; protein phosphatase 1 regulatory inhibitor subunit 1B; Protein phosphatase 1 regulatory subunit 1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32)

Database Links

UniProt ID: Q9UD71

Entrez-Gene Id: 84152

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For Research Use Only. Not for Use in Diagnostic Procedures.

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