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Phospho-Estrogen Receptor α (Ser167) Antibody #2514

Inquiry Info. # 2514

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    Product Specifications

    REACTIVITY
    SENSITIVITY Transfected Only
    MW (kDa) 66
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-Estrogen Receptor α (Ser167) Antibody detects transfected levels of ER alpha only when phosphorylated at serine 167. This antibody does not cross-react with phosphorylated estrogen receptor isoform beta.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser167 of human estrogen receptor alpha. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4).

    Alternate Names

    DKFZp686N23123; E2 receptor alpha; ER; ER-alpha; Era; ESR; ESR1; ESRA; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor alpha delta 3; estrogen receptor alpha delta 4; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; Nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha

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