Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 125 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Phospho-FAK (Tyr397) (D20B1) Rabbit mAb detects FAK protein only when phosphorylated at Tyr397. This antibody may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR.
Species Reactivity:
Human
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Mouse, Rat, Monkey, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK protein.
Background
Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).
- Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
- Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
- Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
- Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
- Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
- Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
- Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.
Limited Uses
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