Cell Signaling Technology Logo - Extra Large

Phospho-FHIT (Tyr114) Antibody #4701

Inquiry Info. # 4701

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 16
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-FHIT (Tyr114) Antibody detects endogenous levels of FHIT only when phosphorylated at tyrosine 114.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr114 of human FHIT. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The fragile histidine triad (FHIT) tumor suppressor gene is frequently altered in human tumors (1). Adenovirus-mediated FHIT gene transfer has been shown to arrest tumor cell growth and induce apoptosis (2,3), and FHIT-/- knockout mice have a higher incidence of spontaneous tumor formation (4,5). FHIT physically associates with ubiquitin-conjugating enzyme 9 (6), and Src has been reported to phosphorylate Tyr114 of FHIT in vitro and in vivo (7). FHIT is a diadenosine P1,P3-triphosphate hydrolase (8), but a His96Asn mutation, which reduces FHIT enzymatic activity by three-fold, does not reduce FHIT tumor suppressor activity (8,9).

    Alternate Names

    AP3A hydrolase; AP3Aase; Bis(5'-adenosyl)-triphosphatase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; FHIT; FRA3B; fragile histidine triad diadenosine triphosphatase; fragile histidine triad gene; Fragile histidine triad protein; tumor suppressor protein

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.