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98074
Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (BSA and Azide Free) #98074

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  1. WB
  2. IHC
Western Blotting Image 1: Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated (+) with λ phosphatase and calf intestinal alkaline phosphatase (CIP), using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (upper) or Glycogen Synthase (15B1) Rabbit mAb #3886 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma, untreated (left) or λ phosphatase treated (right), using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 98074
Cat. # Size Qty. Price
98074SF
100 µg
$ 788

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 85-90
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #47043. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #47043

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of both muscle and liver isoforms of glycogen synthase protein only when phosphorylated at Ser641.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Ser641 of human liver glycogen synthase.

Background

Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver (1). Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals: muscle isoform (glycogen synthase 1, GYS1) and liver isoform (glycogen synthase 2, GYS2), respectively (1). Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649, and Ser653), inhibiting its activity (2,3). Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth (1).
  1. Favaro, E. et al. (2012) Cell Metab 16, 751-64.
  2. Mora, A. et al. (2005) FEBS Lett 579, 3632-8.
  3. Jensen, J. et al. (2012) Am J Physiol Endocrinol Metab 303, E82-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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