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Phospho-GRB10 (Tyr67) Antibody #3701

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Inquiry Info. # 3701

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 76 (Transfected only)
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-GRB10 (Tyr67) Antibody detects transfected GRB10 only when phosphorylated at tyrosine 67. This antibody does not cross-react with phosphorylated GRB7 or GRB14.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr67 of human GRB10. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS, and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules including Raf, Akt, and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4).

    Alternate Names

    Grb-10; GRB-IR; GRB10; GRB10 adapter protein; GRB10 adaptor protein; GRBIR; growth factor receptor bound protein 10; Growth factor receptor-bound protein 10; Insulin receptor-binding protein Grb-IR; IRBP; KIAA0207; maternally expressed gene 1; MEG1; RSS

    For Research Use Only. Not for Use in Diagnostic Procedures.
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