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Phospho-GSK-3alpha/beta (Ser21/9) Antibody

Phospho-GSK-3alpha/beta (Ser21/9) Antibody #5080

Inquiry Info. # 5080

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	45, 51
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-GSK-3alpha/beta (Ser21/9) Antibody can be used in high throughput kinase assays and drug discovery applications. This antibody detects peptides derived from GSK-3a phosphorylated at Ser21 or GSK-3b phosphorylated at Ser9.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of human GSK-3alpha.( Antibodies are purified by protein A and peptide affinity chromatography.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
Description: This antibody is formulated in PBS (no BSA/no glycerol) and quality controlled for use in ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists.

Please direct inquiries for bulk pricing of this antibody, peptide substrates, or protocols to [email protected].

Alternate Names

DKFZp686D0638; glycogen synthase kinase 3 alpha; glycogen synthase kinase 3 beta; Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3 alpha; GSK-3 beta; GSK3A; GSK3alpha; GSK3B; GSK3beta; GSK3beta isoform; Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B

Database Links

UniProt ID: P49840 , P49841

Entrez-Gene Id: 2931 , 2932

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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