Phospho-IFNAR1 (Ser535) (F4V7F) Rabbit Monoclonal Antibody #37851
- WB
- IP
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Phosphorylation of IFNAR1, triggered by ligand binding and ligand-independent means, plays an important role in regulating IFN-I signaling. IFNAR1 phosphorylation at Ser535 leads to endocytosis and IFNAR1 degradation, leading to the decreased sensitivity of cells to subsequent cytokine stimulation (9-11). PKD2 phosphorylates IFNAR1 at Ser535 and is dependent on TYK2 catalytic activity in the ligand-induced pathway (10). In the ligand-independent pathway, IFNAR1 can be phosphorylated at Ser535 by CK1a (12).
- Bazan, J.F. (1990) Proc Natl Acad Sci U S A 87, 6934-8.
- Shuai, K. et al. (1992) Science 258, 1808-12.
- Novick, D. et al. (1994) Cell 77, 391-400.
- Cohen, B. et al. (1995) Mol Cell Biol 15, 4208-14.
- Platanias, L.C. (2005) Nat Rev Immunol 5, 375-86.
- de Weerd, N.A. and Nguyen, T. (2012) Immunol Cell Biol 90, 483-91.
- Trinchieri, G. (2010) J Exp Med 207, 2053-63.
- Zanin, N. et al. (2020) Front Immunol 11, 615603.
- Marijanovic, Z. et al. (2006) Biochem J 397, 31-8.
- Zheng, H. et al. (2011) Mol Cell Biol 31, 710-20.
- Liu, J. et al. (2009) Cell Host Microbe 5, 72-83.
- Bhattacharya, S. et al. (2010) J Biol Chem 285, 2318-25.
Alternate Names
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; IFN-alpha-REC; IFN-alpha/beta receptor 1; IFN-R-1; IFNAR; IFNAR1; IFNBR; IFRC; INAR1; interferon (alpha, beta and omega) receptor 1; interferon alpha and beta receptor subunit 1; Interferon alpha/beta receptor 1; interferon receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1
Limited Uses
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