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6113
Phospho-IGF-I Receptor β (Tyr1316) Antibody

Phospho-IGF-I Receptor β (Tyr1316) Antibody #6113

This product is discontinued

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Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Phospho-IGF-I Receptor β (Tyr1316) Antibody detects endogenous levels of IGF-I receptor only when phosphorylated at Tyr1316. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1316 of human IGF-I receptor. Antibodies are purified by protein A and peptide affinity chromatography.

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).

Phosphorylation of IGF-I receptor on Tyr1346 (equivalent to Tyr1316 in mature protein) was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (9). Phosphorylation of IGF-I receptor on Tyr1346 was also reported by several other labs in select carcinoma cell lines (10,11).

  1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
  2. Baserga, R. (2000) Oncogene 19, 5574-81.
  3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
  4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
  5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
  6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
  7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
  8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.
  9. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
  10. Peterson, J.E. et al. (1996) J Biol Chem 271, 31562-71.
  11. Knowlden, J.M. et al. (2005) Endocrinology 146, 4609-18.
Entrez-Gene Id
3480
Swiss-Prot Acc.
P08069
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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