Cell Signaling Technology Logo - Extra Large

Phospho-IKKα (Ser180)/IKKβ (Ser181) Antibody #2681

Inquiry Info. # 2681

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY
    SENSITIVITY Endogenous
    MW (kDa) 85 (IKKalpha). 87 (IKKbeta).
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-IKKα (Ser180)/IKKβ (Ser181) Antibody detects IKKα and IKKβ only when phosphorylated at serine 180 or serine 181, respectively.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser180 of human IKKα. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).

    Alternate Names

    CHUK; component of inhibitor of nuclear factor kappa B kinase complex; conserved helix-loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; FLJ40509; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKBKB; IKK-A; IKK-a kinase; IKK-alpha; IKK-B; IKK-beta; IKK1; IKK2; IKKA; IKKB; IMD15; IMD15A; IMD15B; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa B kinase subunit beta; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; TCF-16; TCF16; Transcription factor 16

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.