Buy a SimpleChIP kit and receive a sample of our new Universal qPCR Master Mix* | Learn More >>
8338
Phospho-Insulin/IGF Receptor Antibody Sampler Kit

Phospho-Insulin/IGF Receptor Antibody Sampler Kit #8338

Western Blotting Image 1

Western blot analysis of extracts from MCF7 cells, untreated or stimulated with IGF-I, using Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb (upper) and IGF-I Receptor β Antibody #3027 (lower).

Learn more about how we get our images
Western Blotting Image 2

Western blot analysis of extracts from 293 cells, untreated or IGF-I-treated (100 nM for 2 minutes), using Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody (upper) or control IGF-I Receptor antibody (lower).

Learn more about how we get our images
Western Blotting Image 3

Western blot analysis of untreated and IGF-treated Hela cell extracts as well as untreated and insulin-treated H-4-II-E cell extracts using Phospho-IGF-I-Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151)(19H7) Rabbit mAb

Learn more about how we get our images
Western Blotting Image 4

Western blot analysis of extracts from MCF-7 cells, untreated or IGF-I-treated, using Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb (upper) and IGF-I Receptor β (111A9) Rabbit mAb #3018 (lower).

Learn more about how we get our images
Western Blotting Image 5

Western blot analysis of extracts from various cell lines using Insulin Receptor β (4B8) Rabbit mAb.

Learn more about how we get our images
Western Blotting Image 6

Western blot analysis of extracts from 293 (IGF-I receptor β+) and SK-UT-1 (IGF-I receptor β-) cells using IGF-I Receptor β (D23H3) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).

Learn more about how we get our images
Western Blotting Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Learn more about how we get our images
Western Blotting Image 8

Western blot analysis of extracts from 3T3-L1 adipocytes, untreated or insulin-treated (100 nM for the indicated times), using Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody (upper) or control IR antibody (lower).

Learn more about how we get our images
Western Blotting Image 9

Phospho-IGF-I Receptor

β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb specifically binds to tyrosine phosphorylated IGF-1 and insulin receptors, but not other phosphorylated tyrosine kinases. Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β(Tyr1150/1151) (19H7) Rabbit mAb (upper) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower).

Learn more about how we get our images
IP Image 10

Immunprecipitation of Insulin Receptor beta from insulin treated mIMCD-3 cell extracts using Insulin Receptor beta antibody (Lane 1) Lane 2: No antibody control. Lane 3: Input control.

Learn more about how we get our images
Flow Cytometry Image 11

Flow cytometric analysis of SK-UT-1 cells (blue) and MCF7 cells (green) using IGF-I Receptor β (D23H3) XP® Rabbit mAb.

Learn more about how we get our images
IF-IC Image 12

Confocal immunofluorescent analysis of MCF7 (left) and SK-UT-1 (right) cells using IGF-I Receptor β (D23H3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb 3918 20 µl
  • WB
H M R 95 Rabbit IgG
Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody 3021 20 µl
  • WB
  • IP
H M R 95 Rabbit 
Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb 3024 20 µl
  • WB
H M R 95 Rabbit IgG
Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb 4568 20 µl
  • WB
H M R 95 Rabbit IgG
Insulin Receptor β (4B8) Rabbit mAb 3025 20 µl
  • WB
  • IP
H M R 95 Rabbit IgG
IGF-I Receptor β (D23H3) XP® Rabbit mAb 9750 20 µl
  • WB
  • IP
  • IF
  • F
H M R Mk 95 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Phospho-Insulin/IGF Receptor Antibody Sampler Kit provides an economical means of evaluating total Insulin Receptor and IGF-I Receptor β protein levels as well as Insulin and IGF-I Receptor β phosphorylated at specific sites. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Each antibody in the Phospho-Insulin/IGF Receptor Antibody Sampler Kit recognizes endogenous levels of the specific target protein, with activation state-specific antibodies recognizing target proteins only when phosphorylated at the indicated residues. IGF-I Receptor ß (D23H3) XP® Rabbit mAb detects endogenous levels of total IGF-I receptor ß protein. This antibody does not cross-react with insulin receptor.Phospho-IGF-I Receptor beta (Tyr1135) (DA7A8) Rabbit mAb detects endogenous levels of IGF-I receptor only when phosphorylated at Tyr1135. This antibody cross-reacts with Tyr1150 of insulin receptor and may also cross-react with other overexpressed related tyrosine-phosphorylated tyrosine kinases. Phospho-IGF-I Receptor ß (Tyr1131)/Insulin Receptor ß (Tyr1146) Antibody detects endogenous levels of Tyr1131-phosphorylated IGF-I receptor and Tyr1146-phosphorylated insulin receptor. This antibody cross-reacts with activated PDGF, FGF and EGF receptors; ErbB2; and c-Met. Phospho-IGF-I Receptor ß (Tyr1135/1136)/Insulin Receptor ß (Tyr1150/1151) (19H7) Rabbit mAb detects endogenous levels of IGF-I receptor and insulin receptor only when phosphorylated at tyrosine 1135/1136 or tyrosine 1150/1151, and does not cross-react with other related tyrosine-phosphorylated tyrosine kinases. Phospho-IGF-I Receptor ß (Tyr980) (C14A11) Rabbit mAb detects endogenous levels of IGF-I receptor ß protein only when phosphorylated at Tyr980, and may cross-react with activated insulin receptors and FLT3. Insulin Receptor ß (4B8) Rabbit mAb detects endogenous levels of total insulin receptor ß and does not cross-react with IGF-I receptor ß.

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human IGF-IR β or residues surrounding Tyr960 of human insulin receptor β. Activation state-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1135 of human IGF-I Receptor β, Tyr1135/1136 of human IGF-I Receptor β, or Tyr980 of human IGF-I Receptor β. Activation state-specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues of human IGF-I Receptor β. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).

  1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
  2. Baserga, R. (2000) Oncogene 19, 5574-81.
  3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
  4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
  5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
  6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
  7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
  8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.
Entrez-Gene Id
3480
Swiss-Prot Acc.
P08069
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Upstream / Downstream

pathwayImage

Explore pathways related to this product.