Products /
Primary Antibodies /
Phospho-IRAK1 (Thr209) Antibody

Phospho-IRAK1 (Thr209) Antibody #4986

Inquiry Info. # 4986

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	81 to 84
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-IRAK1 (Thr209) Antibody detects endogenous levels of IRAK1 only when phosphorylated at threonine 209.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr209 of human IRAK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).
Upon IL-1 stimulation, IRAK-1 regulates its function as an adaptor molecule by sequential autophosphorylation events on various domains. Thr209 appears to be the first autophosphorylation site in the sequence (4).

Alternate Names

interleukin 1 receptor associated kinase 1; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; IRAK1; pelle; Pelle homolog

Database Links

UniProt ID: P51617

Entrez-Gene Id: 3654

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For Research Use Only. Not for Use in Diagnostic Procedures.

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