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  3. Phospho-MEK1/2 (Ser217/221) (166F8) Rabbit Monoclonal Antibody

Phospho-MEK1/2 (Ser217/221) (166F8) Rabbit Monoclonal Antibody #2354

Inquiry Info. # 2354

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 45
    Source/Isotype Rabbit IgG

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-MEK1/2 (Ser217/221) (166F8) Rabbit Monoclonal Antibody detects endogenous levels of MEK1/2 only when phosphorylated at serine 217/221.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser217/221 of human MEK1/2.

    Background

    MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

    Alternate Names

    CFC3; CFC4; Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 1; ERK activator kinase 2; FLJ26075; MAP kinase kinase 1; MAP kinase kinase 2; MAP2K1; MAP2K2; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MAPKK1; MAPKK2; MEK 1; MEK 2; MEK1; MEK2; MEL; mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; MKK2; MP2K1; MP2K2; PRKMK1; PRKMK2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

    Database Links

    UniProt ID: P36507 , Q02750


    Entrez-Gene Id: 5605 , 5604


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