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Phospho-MEK1/2(Ser217/221) Antibody

Phospho-MEK1/2(Ser217/221) Antibody #5130

Inquiry Info. # 5130

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	45
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-MEK1/2(Ser217/221) Antibody can be used in high throughput kinase assays and drug discovery applications. This antibody detects peptides derived from MEK1/2 phosphorylated at Ser217/221.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser217/221 of human MEK1/2.( Antibodies are purified by protein A and peptide affinity chromatography.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
Description: This antibody is formulated in PBS (no BSA/no glycerol) and quality controlled for ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists.
Please direct inquiries for bulk pricing of this antibody, peptide substrates, or protocols to [email protected].

Alternate Names

CFC3; CFC4; Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 1; ERK activator kinase 2; FLJ26075; MAP kinase kinase 1; MAP kinase kinase 2; MAP2K1; MAP2K2; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MAPKK1; MAPKK2; MEK 1; MEK 2; MEK1; MEK2; MEL; mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; MKK2; MP2K1; MP2K2; PRKMK1; PRKMK2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Database Links

UniProt ID: P36507 , Q02750

Entrez-Gene Id: 5605 , 5604

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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