Phospho-MFF Isoform 5 (Ser146) (F5U3I) Rabbit mAb #36177
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 27 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Research studies have demonstrated that the ability of MFF to drive acute mitochondrial fission in response to mitochondrial stress is controlled by AMPK-dependent phosphorylation. AMPK directly phosphorylates MFF at two sites, which allow for enhanced recruitment of Drp1 to the mitochondria (7). Multiple isoforms of MFF are expressed as a result of alternative splicing (8). One of the major MFF phosphorylation sites (Ser172 of human isoform 1/Ser146 of human isoforms 2-5) lies within an AMPK phosphorylation motif that spans the boundary of differentially spliced MFF isoform exons, suggesting that AMPK phosphorylates MFF splice isoforms to varying degrees.
- Liu, R. and Chan, D.C. (2015) Mol Biol Cell 26, 4466-77.
- Shen, Q. et al. (2014) Mol Biol Cell 25, 145-59.
- Losón, O.C. et al. (2013) Mol Biol Cell 24, 659-67.
- Otera, H. et al. (2010) J Cell Biol 191, 1141-58.
- Itoyama, A. et al. (2013) Biol Open 2, 998-1006.
- Gandre-Babbe, S. and van der Bliek, A.M. (2008) Mol Biol Cell 19, 2402-12.
- Toyama, E.Q. et al. (2016) Science 351, 275-281.
- Ducommun, S. et al. (2015) Cell Signal 27, 978-88.
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