Phospho-MFF (Ser146) Antibody #49281
- WB
Inquiry Info. # 49281
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Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25, 27 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Research studies have demonstrated that the ability of MFF to drive acute mitochondrial fission in response to mitochondrial stress is controlled by AMPK-dependent phosphorylation. AMPK directly phosphorylates MFF at two sites to allow for enhanced recruitment of Drp1 to the mitochondra (7). Multiple isoforms of MFF are expressed as a result of alternative splicing (8). One of the major phosphoacceptor sites of MFF (Ser172 of human isoform 1/Ser146 of human isoforms 2-5) lies within an AMPK phsophorylation motif that spans the boundary of differentially spliced exons of MFF isoforms, suggesting that MFF splice isoforms are phosphorylated by AMPK to varying degrees.
- Liu, R. and Chan, D.C. (2015) Mol Biol Cell 26, 4466-77.
- Shen, Q. et al. (2014) Mol Biol Cell 25, 145-59.
- Losón, O.C. et al. (2013) Mol Biol Cell 24, 659-67.
- Otera, H. et al. (2010) J Cell Biol 191, 1141-58.
- Itoyama, A. et al. (2013) Biol Open 2, 998-1006.
- Gandre-Babbe, S. and van der Bliek, A.M. (2008) Mol Biol Cell 19, 2402-12.
- Toyama, E.Q. et al. (2016) Science 351, 275-81.
- Ducommun, S. et al. (2015) Cell Signal 27, 978-88.
Alternate Names
C2orf33; DKFZp666J168; EMPF2; GL004; MFF; MFF iso2; MFF iso5; MGC110913; Mitochondrial fission factor
Limited Uses
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