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3065
Phospho-PDK1 (Tyr373/376) Antibody
Primary Antibodies

Phospho-PDK1 (Tyr373/376) Antibody #3065

Reviews ()
Citations (2)

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 60 to 68
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-PDK1 (Tyr373/376) Antibody detects endogenous levels of PDK1 when only phosphorylated at tyrosine 373/376. The antibody may cross-react with some other proteins. CST recommends an immunoprecipitation of PDK1 prior to Western immunoblotting.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr373/376 of human PDK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.

PDK1 becomes phosphorylated at Tyr373/376 and translocates to the plasma membrane in response to pervanadate or insulin treatment. Mutational analysis of these sites indicates that Tyr373/376 is important for PDK1 catalytic activity (4).

  1. Belham, C. et al. (1999) Curr. Biol. 9, R93-R96.
  2. Toker, A. and Newton, A.C. (2000) Cell 103, 185-188.
  3. Williams, M.R. et al. (2000) Curr. Biol. 10, 439-448.
  4. Park, J. et al. (2001) J Biol Chem 276, 37459-71.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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