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Phospho-PERK (Thr980) Antibody

Phospho-PERK (Thr980) Antibody #3191

Inquiry Info. # 3191

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Product Specifications

REACTIVITY
SENSITIVITY	Endogenous
MW (kDa)	170
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-PERK (Thr980) Antibody detects endogenous levels of PERK phosphorylated at threonine 980.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr980 of mouse PERK. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.

Alternate Names

DKFZp781H1925; E2AK3; EIF2AK3; eukaryotic translation initiation factor 2 alpha kinase 3; Eukaryotic translation initiation factor 2-alpha kinase 3; heme-regulated EIF2-alpha kinase; HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PERK; PRKR-like endoplasmic reticulum kinase; truncated eukaryotic translation initiation factor 2 alpha kinase 3; WRS

Database Links

UniProt ID: Q9NZJ5

Entrez-Gene Id: 9451

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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