Render Target: STATIC
Render Timestamp: 2024-11-01T09:43:12.451Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-30 01:59:50.315
Product last modified at: 2024-09-30T08:00:59.229Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-PFAS (Thr619) (F1L7L) Rabbit mAb #75628

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 145
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-PFAS (Thr619) (F1L7L) Rabbit mAb recognizes endogenous levels of PFAS protein only when phosphorylated at Thr619.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr619 of human PFAS protein.

    Background

    Phosphoribosylformylglycinamidine synthase (PFAS) is a metabolic enzyme (deamidase) associated with a protein complex known as the “purinosome,” which is involved in de novo purine synthesis (1). Research studies have shown that activation of the MAPK/ERK pathway leads to downstream activation of PFAS. Specifically, it was shown that PFAS is a substrate of ERK2, which phosphorylates PFAS at Thr619; activation of PFAS results in the de novo generation of purine intermediates required for cell and tumor growth (2). It has also been reported that both PFAS and viral PFAS-like proteins can activate RIG-I pattern recognition receptors, implicating PFAS-mediated deamidation in the innate immune response to viral infection (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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