Phospho-PLK1 (Thr210) Matched Antibody Pair #45199
- ELISA
Supporting Data
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Formulation
Storage
Product Description
The Phospho-PLK1 (Thr210) Matched Antibody Pair is ideal for use with immunoassay technologies and high-throughput ELISA platforms requiring antibody pairs with specialized or custom antibody labeling. Labels include fluorophores, lanthanides, biotin, and beads. Platforms requiring conjugated Matched Antibody Pairs include MSD, Quanterix Simoa, Alpha Technology (AlphaScreen, AlphaLISA, LANCE, HTRF), and Luminex.
Learn how Matched Antibody Pairs move your projects forward, faster at cst-science.com/matched-antibody-pairs.
Background
Substitution of Thr210 with Asp has been reported to elevate PLK1 kinase activity and delay/arrest cells in mitosis, while a Ser137Asp substitution leads to S-phase arrest (12). In addition, while DNA damage has been found to inhibit PLK1 kinase activity, the Thr210Asp mutant is resistant to this inhibition (13). PLK1 has been reported to be phosphorylated in vivo at Ser137 and Thr210 in mitosis; DNA damage prevents phosphorylation at these sites (14).
- Nigg, E.A. (1998) Curr Opin Cell Biol 10, 776-83.
- Toyoshima-Morimoto, F. et al. (2002) EMBO Rep 3, 341-8.
- Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
- Peter, M. et al. (2002) EMBO Rep 3, 551-6.
- Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
- Nakajima, H. et al. (2003) J Biol Chem 278, 25277-80.
- Sumara, I. et al. (2002) Mol Cell 9, 515-25.
- Hauf, S. et al. (2001) Science 293, 1320-3.
- Peters, J.M. (1999) Exp. Cell Res. 248, 339-49.
- Kraft, C. et al. (2003) EMBO J 22, 6598-609.
- Kotani, S. et al. (1998) Mol Cell 1, 371-80.
- Jang, Y.J. et al. (2002) J Biol Chem 277, 44115-20.
- Smits, V.A. et al. (2000) Nat Cell Biol 2, 672-6.
- Tsvetkov, L. and Stern, D.F. (2005) Cell Cycle 4, 166-71.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.