Render Target: STATIC
Render Timestamp: 2024-12-06T11:03:19.089Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-30 01:56:33.840
Product last modified at: 2024-10-11T15:15:08.376Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-PZR (Tyr263) (D6A5) Rabbit mAb #8088

Filter:
  • WB
  • IP
  • IF
  • F

    Supporting Data

    REACTIVITY H M R B
    SENSITIVITY Endogenous
    MW (kDa) 30-50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:400
    Flow Cytometry (Fixed/Permeabilized) 1:6400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-PZR (Tyr263) (D6A5) Rabbit mAb recognizes endogenous levels of PZR protein only when phosphorylated at Tyr263.

    Species Reactivity:

    Human, Mouse, Rat, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr263 of human PZR protein.

    Background

    PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8).
    Upon Con A treatment or H2O2 treatment, two PZR intracellular ITIM tyrosine sites-Tyr241 and Tyr263 are phosphorylated (4,8). Phosphorylation of these two sites facilitates recruitment of SHP-2 to PZR which alters the phosphatase activity of SHP-2 and affects its downstream signaling (5,8,9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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