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Phospho-Rad17 (Ser645) Antibody #3421

Inquiry Info. # 3421

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    Product Specifications

    REACTIVITY
    SENSITIVITY Endogenous
    MW (kDa) 80
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-Rad17 (Ser645) Antibody detects endogenous levels of hRad17 only when phosphorylated at serine 645. This antibody does not cross-react with Rad17 phosphorylated at other sites.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser645 of human Rad17. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The human checkpoint protein Rad17 and its fission and budding yeast orthologues (Schizosaccharomyces pombe Rad17 and Saccharomyces cerevisiae Rad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7).

    Alternate Names

    CCYC; cell cycle checkpoint protein (RAD17); Cell cycle checkpoint protein RAD17; FLJ41520; hRad17; R24L; RAD1 homolog; RAD17; RAD17 checkpoint clamp loader component; RAD17 homolog; RAD17 homolog (S. pombe); Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog

    For Research Use Only. Not for Use in Diagnostic Procedures.
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