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14397
Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb
Primary Antibodies

Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb #14397

Reviews ()
Citations (2)

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 61
Isotype Rabbit 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb recognizes endogenous levels of RIP2 protein only when phosphorylated at Ser176. This antibody also cross-reacts with unidentified proteins of approximately 25 kDa and 38 kDa in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176 of human RIP2 protein.

Background

Receptor Interacting Protein 2 (RIP2) is a serine/threonine kinase with a carboxy-terminal caspase activation and recruitment domain (CARD). Association of RIP2 with the tumor necrosis factor receptor (TNFR) causes activation of NF-κB and induction of apoptosis (1-3). Expression of RIP2 is induced in macrophages upon exposure to bacterial cell wall components, such as LPS. RIP2-deficient mouse models demonstrate that this kinase integrates and transduces signals for both the innate and adaptive immune system (4,5).

The RIP2 Ser176 residue serves as a regulatory autophosphorylation site and can be used to monitor the activation state of RIP2 (6).

  1. Inohara, N. et al. (1998) J Biol Chem 273, 12296-300.
  2. McCarthy, J.V. et al. (1998) J Biol Chem 273, 16968-75.
  3. Thome, M. et al. (1998) Curr Biol 8, 885-8.
  4. Chin, A.I. et al. (2002) Nature 416, 190-4.
  5. Kobayashi, K. et al. (2002) Nature 416, 194-9.
  6. Dorsch, M. et al. (2006) Cell Signal 18, 2223-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

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