Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb (BSA and Azide Free) #27254
- WB
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 72 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
For standard formulation of this product see product #89930
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Phosphorylation of Thr592 by cyclin A2/CDK1 was identified as a regulatory mechanism that controls SAMHD1 activity (7,8). SAMHD1 is phosphorylated in proliferating cells, which inhibits its ability to block HIV infection. In resting cells or in cells treated with PMA (TPA) or IFN-α, SAMHD1 phosphorylation is decreased and cells are refractory to HIV infection (7,8).
- Rice, G.I. et al. (2009) Nat Genet 41, 829-32.
- Laguette, N. et al. (2011) Nature 474, 654-7.
- Hrecka, K. et al. (2011) Nature 474, 658-61.
- Powell, R.D. et al. (2011) J Biol Chem 286, 43596-600.
- Goldstone, D.C. et al. (2011) Nature 480, 379-82.
- Lahouassa, H. et al. (2012) Nat Immunol 13, 223-8.
- Cribier, A. et al. (2013) Cell Rep 3, 1036-43.
- White, T.E. et al. (2013) Cell Host Microbe 13, 441-51.
Limited Uses
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