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9912
Phospho-SAPK/JNK Pathway Antibody Sampler Kit

Phospho-SAPK/JNK Pathway Antibody Sampler Kit #9912

Western Blotting Image 1

Western blot analysis of extracts from 293 cells, untreated or UV-treated, NIH/3T3 cells, untreated or UV-treated and C6 cells, untreated or anisomycin-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines, untreated or treated with sorbitol or UV, as indicated, using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from untreated or UV-treated COS cells, NIH/3T3 cells and C6 cells, using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (upper), or ATF-2 (20F1) Rabbit mAb #9226 (lower).

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Western Blotting Image 4

Western blot analysis of extracts from untreated or anisomycin-treated C6 cells, or untreated or UV-treated 293 cells, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb (upper) or c-Jun (60A8) Rabbit mAb #9165 (lower).

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Western Blotting Image 5

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IHC-P (paraffin) Image 6

Immunohistochemical analysis of paraffin-embedded 293T cells untreated (left) or UV-treated (right) using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb.

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Flow Cytometry Image 7

Flow cytometric analysis of HT-29 cells, untreated (blue) or UV-treated (green), using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb compared to a nonspecific negative control antibody (red).

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Flow Cytometry Image 8

Flow cytometric analysis of THP-1 cells, untreated (blue) or Anisomycin treated (green), using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb compared to a nonspecific negative control antibody (red).

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb in the presence of control peptide (left) or Phospho-c-Jun (Ser63) II Blocking Peptide (#1020) (right).

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IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb in the presence of control peptide (left) or Phospho-SAPK/JNK (Thr183/Tyr185) Blocking Peptide #1215 (right).

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human breast carcinoma untreated (left) or lambda phosphatase-treated (right), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cell pellets, control (left) or anisomycin-treated (right), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.

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IHC-P (paraffin) Image 14

Immunohistochemical analysis of paraffin-embedded human skin (normal adjacent to hemangioma), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb 4668 20 µl
  • WB
  • IP
  • IHC
H M R Dm Sc 46, 54 Rabbit IgG
Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb 4514 20 µl
  • WB
  • F
H M R Mk 44 Rabbit 
Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb 5112 20 µl
  • WB
  • F
H M R Mk 70 Rabbit IgG
Phospho-c-Jun (Ser63) (54B3) Rabbit mAb 2361 20 µl
  • WB
  • IHC
H M R 48 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Phospho-SAPK/JNK Pathway Antibody Sampler Kit provides a fast and economical means of evaluating multiple members of the SAPK/JNK pathway as well as their activation state. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Each antibody in the Phospho-SAPK/JNK Antibody Sampler Kit recognizes endogenous levels of the phosphorylated form of its specific target.

Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptides corresponding to residues surrounding Ser257 of human SEK1, Thr183/Tyr185 of human SAPK, Thr71 of human ATF-2 or Ser63 of human c-Jun.

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

  1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
  2. Ichijo, H. (1999) Oncogene 18, 6087-93.
  3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
  4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
  5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
  6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
Entrez-Gene Id
1386 , 3725 , 6416 , 5599
Swiss-Prot Acc.
P15336 , P05412 , P45985 , P45983
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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