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9912
Phospho-SAPK/JNK Pathway Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Phospho-SAPK/JNK Pathway Antibody Sampler Kit #9912

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Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 1

Immunohistochemical analysis of paraffin-embedded 293T cells untreated (left) or UV-treated (right) using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb.

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 2

Flow cytometric analysis of HT-29 cells, untreated (blue) or UV-treated (green), using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb compared to a nonspecific negative control antibody (red).

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 3

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb in the presence of control peptide (left) or Phospho-c-Jun (Ser63) II Blocking Peptide (#1020) (right).

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 4

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 5

Flow cytometric analysis of THP-1 cells, untreated (blue) or Anisomycin treated (green), using Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) (A8J7P) Rabbit mAb compared to a nonspecific negative control antibody (red).

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 6

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb in the presence of control peptide (left) or Phospho-SAPK/JNK (Thr183/Tyr185) Blocking Peptide #1215 (right).

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 7

Western blot analysis of extracts from various cell lines, untreated or treated with sorbitol or UV, as indicated, using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb.

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 8

Immunohistochemical analysis of paraffin-embedded human breast carcinoma untreated (left) or lambda phosphatase-treated (right), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 9

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated with Anisomycin (25 μg/ml, 30 min; +), using Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) (A8J7P) Rabbit mAb (upper), ATF-2/ATF-7 (A9G1M) Rabbit mAb #82870 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower)

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 10

Western blot analysis of extracts from 293 cells, untreated or UV-treated, NIH/3T3 cells, untreated or UV-treated and C6 cells, untreated or anisomycin-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb.

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 12

Western blot analysis of extracts from HeLa cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human ATF-2 protein (hATF-2-Myc; +) or a construct expressing Myc-tagged full-length human ATF-7 protein (hATF-7-Myc; +), using Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) (A8J7P) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 13

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cell pellets, control (left) or anisomycin-treated (right), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded human skin (normal adjacent to hemangioma), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.

Phospho-SAPK/JNK Pathway Antibody Sampler Kit: Image 15

Western blot analysis of extracts from untreated or anisomycin-treated C6 cells, or untreated or UV-treated 293 cells, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb (upper) or c-Jun (60A8) Rabbit mAb #9165 (lower).

To Purchase # 9912T
Product # Size Price
9912T
1 Kit  (4 x 20 µl) $ 327

Product Description

The Phospho-SAPK/JNK Pathway Antibody Sampler Kit provides a fast and economical means of evaluating multiple members of the SAPK/JNK pathway as well as their activation state. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Specificity / Sensitivity

Each antibody in the Phospho-SAPK/JNK Antibody Sampler Kit recognizes endogenous levels of the phosphorylated form of its specific target.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptides corresponding to residues surrounding Ser257 of human SEK1, Thr183/Tyr185 of human SAPK, Thr71 of human ATF-2 or Ser63 of human c-Jun.

Background

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

  1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
  2. Ichijo, H. (1999) Oncogene 18, 6087-93.
  3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
  4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
  5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
  6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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