Phospho-SHP-2 (Tyr580) Antibody

Inquiry Info. # 3754

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	72
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-SHP-2 (Tyr580) Antibody detects endogenous levels of SHP-2 only when phosphorylated at tyrosine 580.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr580 of human SHP-2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 at p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6).

SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are proposed to relieve basal inhibition and stimulate its tyrosine phosphatase activity (5).

Alternate Names

BPTP3; CFC; JMML; METCDS; MGC14433; NS1; protein tyrosine phosphatase non-receptor type 11; protein tyrosine phosphatase-2; protein tyrosine phosphatase, non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTN11; PTP-1D; PTP-2C; PTP2C; PTPN11; SH-PTP2; SH-PTP3; SHP-2; Shp2; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11

Database Links

UniProt ID: Q06124

Entrez-Gene Id: 5781

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Phospho-SHP-2 (Tyr580) Antibody #3754