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Phospho-Stat3 (Ser727) (6E4) Mouse mAb #5190

Inquiry Info. # 5190

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 79, 86
    Source/Isotype Mouse

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-Stat3 (Ser727) (6E4) Mouse mAb can be used in high throughput kinase assays and drug discovery applications. This antibody detects Stat3 only when phosphorylated at serine 727. This antibody does not appreciably cross-react with the corresponding phosphorylated serines of other Stat proteins.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser727 of mouse Stat3.

    Background

    The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
    Description: Cell Signaling Technology antibody formulated in PBS (no BSA/no glycerol) and quality controlled for use in ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists. Please inquire for bulk pricing of this antibody.

    Alternate Names

    Acute-phase response factor; ADMIO; ADMIO1; APRF; DNA-binding protein APRF; FLJ20882; HIES; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT3

    For Research Use Only. Not for Use in Diagnostic Procedures.
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